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用阿拉善黄鼠(Citellus alsachanicus)鼠疫疫源地的鼠疫菌Pgm~+和Pgm~-株,连续通过阿拉善黄鼠进行传代试验。在传代前后用阿拉善黄鼠测其毒力。Pgm~+株连续传代15代后未发现突变,其主要生化特性、抗原结构和毒力都没有发生改变。而含有0.1～0.3％Pgm~+鼠疫菌个体的Pgm~-株在连续传代5代后即突变为Pgm~+株,且毒力增强。但生化特性和抗原结构仍无改变。纯系Pgm~-株在连续传代8～11代后可突变为Pgm~+株,至20代仍未完全突变为Pgm~+,其他生化特性和抗原结构亦没有改变,但其毒力逐渐增强。作者分析鼠疫菌Pgm细胞突变与阿拉善黄鼠动物鼠疫流行和间歇有密切关系,这对研究该疫源地内鼠疫菌的保存机制及流行病学分析提供了参考资料。
The passage test of Pseudomonas solani Pgm ~ + and Pgm ~ - strains from the plague foci of Citellus alsachanicus was carried out continuously. Before and after passage with Alashan gopher test its virulence. No mutation was found after passage of Pgm ~ + strain continuously for 15 generations, and its main biochemical characteristics, antigen structure and virulence did not change. However, Pgm ~ - strain containing 0.1 ~ 0.3% Pgm ~ + Yersinia pestis mutated to Pgm ~ + strain after passage for five generations continuously, and its virulence increased. However, biochemical properties and antigen structure remain unchanged. The pure Pgm ~ - strain could mutate into Pgm ~ + strain after passaged for 8 ~ 11 generations continuously, yet did not completely mutate to Pgm ~ + till the 20th passage, and other biochemical characteristics and antigenic structure did not change, but its virulence gradually increased . The author analyzed that the mutation of Pgm of Pseudomonas aeruginosa was closely related to the epidemic and intermittence of plague in Alzheimer’s disease rats, which provided references for the research on the mechanism of preservation and epidemiological analysis of plague bacteria in this foci.