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在发现彭泽鲫种群内两个不同雌核发育克隆 (H和L) 的基础上, 对彭泽鲫两个克隆和银鲫(D系)、缩骨鲫、野鲫共 5个鲫鱼品系进行RAPD分析。结果 30个引物中有 22个扩增效果良好, 统计分析表明, 彭泽鲫 (L) 与银鲫 (D) 的遗传相似率最高 (0 909091); 彭泽鲫 (H)、缩骨鲫和野鲫三者间的遗传相似率也较大, 其中彭泽鲫 (H) 和缩骨鲫的相似率 ( 0 862069 ) 高于二者与野鲫的相似率 (分别为0 828571、0 830986)。聚类分析结果也表明, 彭泽鲫 (L) 与银鲫 (D系) 的亲缘关系非常接近, 暗示二者可能具有相同的遗传起源; 彭泽鲫 (H)、缩骨鲫和野鲫三者间的亲缘关系次之, 其中彭泽鲫 (H) 和缩骨鲫的亲缘关系较近, 推测二者可能起源于野鲫; 而彭泽鲫 (L) 与银鲫 (D系 ) 二者与彭泽鲫 (H)、缩骨鲫和野鲫三者之间的亲缘关系较远, 说明它们之间存在明显的遗传分化。
On the basis of finding two different gynogenetic clones (H and L) in the population of Pengze crucian carp, five crucian carp lines from two clones of Pengze crucian carp and silver crucian carp (D line), constrictor crucian carp and wild crucian carp RAPD analysis. Results 22 out of 30 primers had good amplification results. Statistical analysis showed that the genetic similarity between Pengze crucian carp (L) and silver crucian carp (D) was the highest (0 909091), that of Pengze crucian carp (H) The genetic similarity between the wild crucian carp and the wild crucian carp was also larger. The similarities between the Pengze crucian carp (H) and the reduced crucian carp (0 862 069) were higher than those between wild crucian carp and wild crucian carp (0 828 571 and 0 830 986 respectively). The results of cluster analysis also showed that the genetic relationship between Pengze crucian carp (L) and silver crucian carp (D line) was very close, suggesting that the two may have the same genetic origin; the Pengze crucian carp (H) Among them, Pengze crucian carp (H) and crucian carp had a close genetic relationship, suggesting that both may originated from wild crucian carp. However, both Pengze crucian carp (L) and silver crucian carp (D series) And Pengze crucian carp (H), crucian carp and wild crucian carp far between the three, indicating that there is a clear genetic differentiation between them.