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目的:建立同时测定呼伦贝尔产赤芍防治心血管系统疾病有效物质群中芍药苷、羟基芍药苷、芍药内酯苷含量的方法。方法:采用高效液相色谱法。色谱柱为Inertsil ODS-SP(250 mm×4.6 mm,5μm),流动相为乙腈-水溶液(梯度洗脱),流速为1.0ml/min,检测波长为230 nm,进样量为20μl。结果:芍药苷、羟基芍药苷、芍药内酯苷的质量浓度分别在10.00~100.00、1.00~25.00、0.40~10.00μg/ml范围内与各自峰面积积分值呈良好的线性关系(r分别为0.999 5、0.999 1、0.999 2);精密度、稳定性、重复性试验的RSD<2%;平均加样回收率分别为98.17%(RSD=1.14%,n=6)、98.08%(RSD=1.50%,n=6)、98.07%(RSD=1.03%,n=6)。结论:该方法操作简便、快速,结果准确,可用于控制呼伦贝尔产赤芍的质量。
Objective: To establish a method for the simultaneous determination of paeoniflorin, hydroxy paeoniflorin and paeoniflorin in the effective substance group of Hulun Buir-producing red peony root in the prevention and treatment of cardiovascular diseases. Methods: Using high performance liquid chromatography. The chromatographic column was Inertsil ODS-SP (250 mm × 4.6 mm, 5 μm). The mobile phase consisted of acetonitrile-water (gradient elution) with a flow rate of 1.0 ml / min and a detection wavelength of 230 nm. Results: The concentrations of paeoniflorin, paeoniflorin and paeoniflorin showed a good linear relationship with the integral values of their peak areas in the range of 10.00-100.00, 1.00-25.00, 0.40-10.00μg / ml respectively (r = 0.999 (RSD = 1.14%, n = 6), 98.08% (RSD = 1.50 %, n = 6), 98.07% (RSD = 1.03%, n = 6). Conclusion: The method is simple, rapid, accurate and can be used to control the quality of red peony root produced by Hulunbeier.