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目的观察芬太尼对足炎症痛大鼠的抗伤害性感受作用及其作用机制。方法成年雌性SD大鼠加只,随机分为7组。用热测痛仪测定大鼠双后足的基础伤害性感受阈值,然后双后足底皮下注射角叉菜胶建立外周炎症痛模型。炎症6 h(A)、12 h(B)、24 h(C)、72 h(D)组(n=5),足底皮下注射0．5μg/50μl芬太尼;正常对照组(E组)(n=5),足底皮下注射0．5μg／50μl芬太尼;炎症24 h组(F 组)(n=5),足底皮下注射0．5μg芬太尼混合20 μg纳洛酮/50μl;炎症24 h大鼠腹部皮下注射0．5μg/ 50μl芬太尼组(G1组)和等量生理盐水对照组(G2组)(n=5)。以上各组分别在用药前、后测伤害性感受阈值。结果用药前即刻,A组伤害性感受阈最短,B、C、D组伤害性感受阈延长(P<0．05),各组左、右足伤害性感受阈比较差异无统计学意义(P>0．05)。用药后,A、B、C组右足伤害性感受阈与用药前即刻比较延长,三组右足伤害性感受阈与左足比较延长(P<0．05)。结论芬太尼可通过外周阿片受体对外周炎症痛大鼠产生抗伤害性感受作用。
Objective To observe the antinociceptive effects of fentanyl on foot inflammatory rats and its mechanism. Methods Adult female Sprague-Dawley rats were randomly divided into 7 groups. The basal nociceptive thresholds of both hind paws of rats were measured by thermogravimetry, and then the model of peripheral inflammatory pain was established by subcutaneous injection of carrageenan with double hind paw. Infants were injected subcutaneously with 0.5μg / 50μl fentanyl for 6 hours (A), 12 hours (B), 24 hours (C) and 72 hours (n = 5), subcutaneous injection of 0.5μg / 50μl fentanyl in the planta, inflammatory group 24 hours (group F) (n = 5), subcutaneous injection of 0.5μg fentanyl / 50μl; 0.5μg / 50μl fentanyl group (G1 group) and normal saline control group (G2 group) (n = 5) were injected subcutaneously into the abdomen of inflamed 24 h rats. The above groups were measured before and after treatment, nociceptive threshold. Results Immediately before treatment, the threshold of nociceptive sensation in group A was the shortest, and the threshold of nociceptive sensation in groups B, C and D was prolonged (P <0.05). There was no significant difference in the threshold of nociceptive sensation between the two groups (P> 0.05). After treatment, the right threshold of nociceptive sensation in groups A, B and C was prolonged immediately before treatment, and the threshold of right noxious sensation in the three groups was prolonged (P <0.05). Conclusion Fentanyl can exert an antinociceptive effect on peripheral inflammatory pain rats through peripheral opioid receptors.