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目的：观察松质骨基质－ 骨髓基质成骨细胞复合移植皮下成骨作用，以探索松质骨基质作为骨组织工程支架材料的可行性。方法：新生兔骨髓细胞体外培养、诱导后，接种于藻酸盐／松质骨基质中，形成松质骨基质／藻酸盐／骨髓基质成骨细胞复合物，并植入裸鼠背部皮下，对照组单纯植入松质骨基质。植入４、８ 周后行 Ｘ线摄片和组织学检查，观察骨形成情况。结果：松质骨基质／骨髓基质成骨细胞复合物皮下成骨效果明显优于松质骨基质。前者兼有纤维成骨和软骨成骨，以纤维成骨为主；后者仅见少量软骨成骨。结论：松质骨基质可以作为一种良好的骨组织工程支架材料。
OBJECTIVE: To observe the subcutaneous osteogenesis of cancellous bone-marrow stromal osteoblasts in order to explore the feasibility of cancellous bone matrix as a scaffold for bone tissue engineering. Methods: The newborn rabbit bone marrow cells were cultured in vitro. After inoculation, the newborn rabbit bone marrow cells were inoculated into the alginate / cancellous bone matrix to form the cancellous bone matrix / alginate / bone marrow stromal osteoblast complex and subcutaneously implanted into the back of nude mice. The control group was implanted with cancellous bone matrix. 4,8 weeks after implantation, X-ray and histological examination were performed to observe the bone formation. Results: The cancellous bone matrix / bone marrow stromal osteoblast complex subcutaneously osteogenic significantly better than the cancellous bone matrix. The former has both fibroblasts and cartilage osteogenesis, fibrosis mainly; the latter only see a small amount of cartilage osteogenesis. Conclusion: Cancellous bone matrix can be used as a good scaffold for bone tissue engineering.