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为改善已研制光学微扫描显微热成像系统的空间分辩力,微扫描零点需要确定。基于几何原理,研究提出了一种利用数字图像处理技术进行零点定标的方法。给出了微扫描零点的定义、详细分析了零点定标原理及方法,完成了实际显微热成像系统的微扫描零点定标。针对红外热图像,模拟零点定标前后的实际系统,采用不同重构方法进行了仿真研究,给出了评价参数;利用零点定标后的光学微扫描显微热成像系统采集低分辨力显微热图像序列进行过采样重构研究,仿真和实验结果表明了该方法的有效性,从而得到了高分辨力光学微扫描显微热成.像系统,可应用于需要显微热分析的场合。
In order to improve the spatial resolution of the developed optical micro-scanning micro-thermal imaging system, the micro-scanning zero point needs to be determined. Based on the principle of geometry, a method of zero-point calibration using digital image processing technology is proposed. The definition of zero point of micro-scanning is given. The zero-point calibration principle and method are analyzed in detail, and the zero-point scaling of micro-scan of the actual micro-thermal imaging system is completed. According to the infrared thermal image, the actual system before and after the simulation of zero calibration was simulated by different reconstruction methods, and the evaluation parameters were given. The optical micro-scanning micro-thermal imaging system after zero calibration was used to acquire low-resolution microscopy The thermal image sequence is oversampled and reconstructed, and the simulation and experimental results show the effectiveness of this method, resulting in a high-resolution optical micro-scanning micro-thermal imaging system that can be used in applications where micro-thermal analysis is required.