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从ＬＰＳ活化的人外周血单核细胞总ＲＮＡ中，用ＲＴＰＣＲ方法扩增出编码成熟人白细胞介素１５（ｈＩＬ１５）的ｃＤＮＡ，并将其克隆于ｐＢｌｕｅＳｋｍ质粒中，序列测定结果与预期一致。再将ｈＩＬ１５ｃＤＮＡ克隆于表达载体ｐＢＶ２２０，构建了高效表达克隆ｐＢＶＩＬ１５。热诱导４ｈ，ｈＩＬ１５蛋白表达即达高峰。表达的重组蛋白经ＳＤＳＰＡＧＥ及凝胶密度扫描分析，分子量约１３ｋＤ，占菌体总蛋白的３３％，经包涵体提取及ＳｅｐｈａｃｒｙｌＳ１００凝胶过滤纯化，重组蛋白纯度达９５％以上，具有显著的促鼠Ｔ淋巴母细胞增殖作用
The cDNA encoding mature human interleukin 15 (hIL-15) was amplified by RT-PCR from the total RNA of LPS-activated human peripheral blood mononuclear cells and cloned into pBlueSkm plasmid. The results of sequence analysis Expected agreement. Then hIL 15 cDNA was cloned in the expression vector pBV220 to construct a highly expressed clone pBV IL-15. Heat induced 4h, hIL 15 protein expression reached the peak. The expressed recombinant protein by SDS PAGE and gel density scanning analysis, molecular weight of about 13kD, accounted for 33% of the total bacterial protein by inclusion body extraction and SephacrylS 100 gel filtration purification, recombinant protein purity of 95% or more, with Significant stimulation of rat T lymphoblast proliferation