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利用高效液相色谱法,测定了108份水稻种质资源材料子粒中的γ-氨基丁酸含量,分析了不同种质资源材料间γ-氨基丁酸含量差异及与子粒性状的相关性,以γ-氨基丁酸含量差异较大的高粱稻-1与宁农黑粳配制杂交组合,测定亲本、杂交F1及216个F2子粒中的γ-氨基丁酸含量,分析其含量与相对胚重和子粒性状的相关性及变异系数,估算各性状的广义遗传力。结果表明:108份种质资源材料子粒中的γ-氨基丁酸含量变异范围为2.39~12.03 mg/100g,,平均含量为6.30±1.99 mg/100g,变异系数为31.59%;不同水稻种质资源材料子粒中的γ-氨基丁酸含量与粒厚、千粒重呈极显著的负相关;杂交F1子粒γ-氨基丁酸含量为8.39±0.11 mg/100g,介于双亲之间;F2单株子粒中的γ-氨基丁酸含量总体呈偏正态分布,且出现明显的超亲现象,说明水稻子粒中的γ-氨基丁酸含量是由多基因控制的数量性状遗传;杂交F2子粒中的γ-氨基丁酸含量与相对胚重呈极显著的正相关,与粒厚、千粒重呈极显著的负相关,与粒长呈显著的负相关;F2单株子粒中的γ-氨基丁酸含量、粒厚和千粒重的广义遗传力相对较高,分别为98.12%、91.99%、96.37%,在育种中对这些性状可进行早期选择。
The contents of γ-aminobutyric acid in 108 accessions of rice germplasm resources were determined by high performance liquid chromatography (HPLC), and the correlations between γ-aminobutyric acid content and the grain traits among different germplasm resources were analyzed. γ-aminobutyric acid content of hybrid rice sorghum-1 and Ningnong black japonica hybrid combinations prepared parents F1 hybrids and 216 F2 grain gamma-aminobutyric acid content, analysis of its relative germ weight and sub Grain trait correlation and coefficient of variation to estimate the general heritability of each trait. The results showed that the variation of γ-aminobutyric acid content in seeds from 108 germplasm resources was 2.39 ~ 12.03 mg / 100g, the average content was 6.30 ± 1.99 mg / 100g, and the variation coefficient was 31.59% The content of γ-aminobutyric acid in the grain of the material was extremely significantly negatively correlated with grain thickness and thousand-grain weight. The γ-aminobutyric acid content of the hybrid F1 grain was 8.39 ± 0.11 mg / 100g, which was between parents; The γ-aminobutyric acid content of the F2 population was generally normal distribution with obvious trans-parent phenotype, indicating that γ-aminobutyric acid content in rice grains was inherited by quantitative traits controlled by multiple genes. The γ- Aminobutyric acid content was significantly and positively correlated with relative embryo weight, negatively correlated with grain thickness and 1000-grain weight, and negatively correlated with grain length. The content of γ-aminobutyric acid The broad heritability of grain weight and thousand-grain weight was relatively high, which were 98.12%, 91.99% and 96.37%, respectively. Early selection of these traits could be made during breeding.