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膜蛋白P84(SHPS - 1)及其配体IAP(integrinassociatedprotein ,integrin相关蛋白 )均在中枢神经系统及其后神经外组织表达并参与信息传递 ,在小鼠视网膜表达这两个分子的部位主要是视网膜突触所在的内丛状层和外丛状层 ,提示该分子对可能与突触有关。免疫电镜研究的结果证实在外丛状层 ,这两个分子分布在突触的部位 ,原位杂交研究结果表明P84信使核糖核酸 (mRNA)既在内核层也在外核层 (感光细胞层 )表达 ,而IAP只有在内核层的某些细胞中表达 ,感光细胞不表达该分子 ,提示外丛状层突触部位的IAP只有来自内核层的神经元 (即突触后来源 )。在IAP基因缺陷型小鼠视网膜上 ,P84在外丛状层的分布向外核层弥散 ,失去了正常的突触分布特征 ,说明P84的突触分布需要IAP与P84的相互作用。这些研究结果提示 ,P84和IAP在视网膜的信息传递效应可能发生在突触的部位。由于P84在突触的两侧均有分布 ,因而这对突触相关分子代表了一种可能调节突触活动或者神经元相互之间营养作用的所谓双向信息传递系统。
Membrane protein P84 (SHPS - 1) and its ligand IAP (integrin - related protein) are both expressed in the central nervous system and its extranodal tissues and involved in information transmission. The two molecules expressed mainly in the retina of mice Retina synapses where the inner plexiform and outer plexiform layer, suggesting that the molecular pairs may be related to synapses. The results of immunoelectron microscopy confirmed that in the outer plexiform layer, the two molecules are located at the synapse site. In situ hybridization results show that P84 messenger RNA (mRNA) is expressed both in the inner nuclear layer and in the outer nuclear layer (photoreceptor cell layer) However, IAP is only expressed in some cells in the inner nuclear layer, and the photoreceptor cells do not express this molecule, suggesting that only IAPs from the inner nuclear layer (ie, postsynaptic sources) are the IAPs of the extra plexiform layer. In the IAP-deficient mouse retina, the distribution of P84 in the outer plexiform layer diffuses to the outer nuclear layer, losing the normal synaptic distribution, indicating that the synaptic distribution of P84 requires the interaction of IAP and P84. These findings suggest that the informational effects of P84 and IAP on the retina may occur at the synaptic site. Since P84 is distributed on both sides of the synapse, the synapse related molecules represent a so-called two-way information delivery system that may modulate synaptic activity or the nutritional interactions among neurons.