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一氧化氮(NO)除了是一种对抗微生物的潜在性防御因子和自身免疫性疾病的介质之外,它还可能是一种强烈的T细胞应答介质。本文通过检测T细胞对超抗原葡萄球菌肠毒素B(SEB)的反应以研究NO对T细胞功能的影响。实验动物选用BALB/c小鼠(8周龄)。实验1组用NO供体(二硝酸异山梨醇,ISO)处理,剂量为每天150mg/kg,投放于饮水中;实验2组未经ISO处理。两组小鼠均静脉注射50μg SEB,对照组只注射PBS。注
In addition to being a mediator of potential defense against microorganisms and autoimmune diseases, nitric oxide (NO) may also be a potent T cell response mediator. In this study, the effect of NO on T cell function was investigated by detecting T cell responses to superantigen staphylococcal enterotoxin B (SEB). BALB / c mice (8 weeks old) were selected as experimental animals. The rats in experiment 1 were treated with NO donor (isosorbide dinitrate, ISO) in a dose of 150 mg / kg per day and were placed in drinking water. The second experiment group was not treated with ISO. Two groups of mice were intravenous injection of 50μg SEB, the control group only PBS. Note