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目的 调查兰州地区腹泻患者中艰难梭菌的流行特点,揭示国内艰难梭菌感染的现况。方法 通过细胞毒检测试验和酶联免疫吸附试验对206份临床粪便样品进行毒素检测。结果 206份粪便滤液经细胞毒检测有26份样品使非洲绿猴肾细胞(vero细胞)圆缩化,确认含有艰难梭菌毒素;经酶联免疫吸附试验有28份为阳性,其中23份与细胞毒检测结果一致,与细胞毒试验的符合率为88.5%。结论 兰州地区门诊腹泻患者中艰难梭菌感染率约为12.62%。
Objective To investigate the prevalence of Clostridium difficile in patients with diarrhea in Lanzhou and to reveal the status of C. difficile infection in China. Methods Toxin was detected in 206 clinical stool samples by cytotoxicity test and enzyme-linked immunosorbent assay. Results Twenty-six samples of 206 fecal filtrate were examined for cytotoxicity to round out vero cells of African green monkey, confirming the presence of C. difficile toxin; 28 were positive by enzyme-linked immunosorbent assay, 23 of which Cytotoxicity test results were consistent with the cytotoxicity test was 88.5%. Conclusion Clostridium difficile infection rate in outpatient diarrhea in Lanzhou is about 12.62%.