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目的探讨柞蚕雄蛾提取液对放疗引起的免疫抑制的调节作用及其机制。方法50只Wistar雄性大鼠随机分为对照组、单纯照射组和柞蚕雄蛾提取液大、中、小剂量治疗组,于治疗后第14天观察各组胸腺、脾脏指数变化,流式细胞术检测大鼠外周血细胞中淋巴细胞亚型改变,免疫组化检测大鼠脾脏中Th1类细胞因子IL-2、IFN-γ和Th2类细胞因子IL-4、IL-10表达变化。结果单纯照射组与对照组相比,胸腺、脾脏指数下降,外周血细胞中CD3~+、CD4~+淋巴细胞明显减少,脾脏中IL-4、IL-10表达增加,差异有极显著意义(P＜0．01),IL-2、IFN-γ表达元明显改变。大剂量治疗组与单纯照射组相比胸腺、脾脏指数增加,外周血细胞中CD3~+、CD4~+淋巴细胞升高,差异有极显著意义(P＜0．01),脾脏中IL-2、IFN-γ表达增加,IL-4、IL-10表达下降(P＜0．01)。结论柞蚕雄蛾提取液可以有效降低放疗引起的免疫抑制,促进T细胞增殖,诱导Th1类细胞因子的表达,促进Th2类细胞因子向Th1类细胞因子漂移。
Objective To investigate the regulation of immunosuppression induced by radiotherapy in male tussah extract and its mechanism. Methods Fifty Wistar male rats were randomly divided into control group, irradiation group and male and female tussah large, medium and small dose treatment groups, the index of thymus and spleen in each group was observed on the 14th day after treatment. Flow cytometry The changes of lymphocyte subsets in rat peripheral blood were detected. The expressions of Th1 cytokines IL-2, IFN-γ and Th2 cytokines IL-4 and IL-10 in rat spleen were detected by immunohistochemistry. Results Compared with the control group, the thymus and spleen indices of pure irradiation group decreased, but the numbers of CD3 ~ + and CD4 ~ + lymphocytes in peripheral blood cells decreased significantly. The expressions of IL-4 and IL-10 in spleen increased significantly <0.01), IL-2, IFN-γ expression significantly changed. In the high-dose treatment group, the thymus and spleen indexes increased compared with those in the control group, and the levels of CD3 ~ + and CD4 ~ + lymphocytes in the peripheral blood cells were significantly increased (P <0.01) IFN-γ expression increased, IL-4, IL-10 expression decreased (P <0.01). Conclusion The extract of male tussah moth can effectively reduce the immunosuppression induced by radiotherapy, promote the proliferation of T cells, induce the expression of Th1 cytokines and promote the migration of Th2 cytokines to Th1 cytokines.