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用决明（ＣａｓｉａｏｂｔｕｓｉｆｏｌｉａＬ．）子叶和下胚轴为材料，游离和培养原生质体。结果表明，１５日龄无菌苗的子叶和下胚轴比较适于游离原生质体，每１ｇ材料的原生质体产量高达１．６×１０４个；ＰｅｃｔｏｌｙａｓｅＹ－２３是分离原生质体所必需的；用含２，４－Ｄ０．４ｍｇ／Ｌ（以下单位同），ＮＡＡ１．０与ＫＴ０．１的ＫＭ８Ｐ漂浮培养利于原生质体的分裂。培养４ｄ后，原生质体开始分裂，１５ｄ后其植板率约为１９．２％，３０ｄ形成了细胞团或小愈伤组织。增殖愈伤组织在分化培养基上培养，可分化出芽。芽在生根培养基上培养１４ｄ生根，从而再生决明小植株。
Protoplasts were isolated and cultured using cotyledons and hypocotyls of Casia obtusifolia L. as materials. The results showed that the cotyledons and hypocotyls of 15-day-old sterile seedlings were suitable for free protoplasts, and the yield of protoplast per 1g material was as high as 1.6 × 104. PectolyaseY-23 was necessary for the isolation of protoplasts. 2,4-D0.4mg / L (the same units below), NAA1.0 and KT0.1 KM8P floating culture conducive to protoplast division. After 4 days of culture, the protoplasts began to divide. After 15 days, their protoplast rate was about 19.2%. After 30 days, cell masses or small callus were formed. Proliferation callus cultured on differentiation medium can differentiate buds. Buds in the rooting medium for 14 days after rooting, regeneration Cassia small plants.