以属于我国南方分布的普通野生稻(Oryza sativa L.f.spontanea)的红芒野生稻和藤桥野生稻作母本,与栽培稻不同品种作父本杂交和连续回交,进行核代换,培育出了相应的雄性不育系。并已获得相应的恢复系。在选育三系的过程中证明我国南方的普通野生稻在细胞质的生理和遗传特性上存在着不同类型,对三系选育有不同的利用价值。 从有关的遗传学及细胞学研究说明这种雄性不育性不是一种简单的遗传特性,而是牵涉到一系列遗传、生理特性的异常,有着复杂的遗传基础。不育性和恢复性表现出类似于数量性状的遗传行为。作者认为雄性不育性是参加核代换亲本的遗传基础相互作用的结果,是远缘核质不亲和性的表现。当远缘的核质结合时产生雄性不育,而近缘的核质结合时造成花粉育性的恢复,因此三系配套与亲本亲缘的远近密切相关,从而批判了旧的三系理论及其所设想的育种方法。 Breeding male wild rice (Oryza sativa L.) and wild rattan (Oryza sativa L.) wild rice belonging to Oryza sativa Lfspontanea distributed in the south of our country as the male parent and different cultivated rice as male parents and backcrossing continuously, The corresponding male sterile line. And have access to the appropriate recovery system. In the process of breeding three lines, it has been proved that there are different types of common wild rice in southern China in terms of the physiological and genetic characteristics of the cytoplasm and different utilization values ​​for the three-line selection. From the relevant genetic and cytological studies show that this male sterility is not a simple genetic characteristics, but involves a series of genetic and physiological abnormalities, with a complex genetic basis. Infertility and restitution demonstrated genetic behavior similar to quantitative traits. The authors consider male sterility as a result of the genetic basis of participation in nuclear substitution parents and a manifestation of distant incompatibility of the cytoplasm. When the distant nuclei and cytoplasm are combined, male sterility is generated, and the combination of proximal nuclear and cytoplasm results in the restoration of pollen fertility. Therefore, the relationship between the three lines and their relatives is closely related, thus criticizing the old three-line theory and its The conceived breeding method.