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背景溃疡性结肠炎发病率呈逐年上升趋势,但发病机制不明,众多基于基因水平的研究不能完全阐明蛋白质水平的变化,差异蛋白质组学是从蛋白质水平研究溃疡性结肠炎发病机制的新方法。目的运用蛋白组学方法探讨溃疡性结肠炎的发病机制。方法选取12例活动期溃疡性结肠炎患者及12例正常肠黏膜为研究对象,提取组织蛋白做2-D电泳,银染,胶图分析找差异点,质谱鉴定蛋白质,最后Western-blot验证差异点。结果差异蛋白组学共鉴定出蛋白质30个(上调16个,下调14个),成功选取其中的vimentin,并得到Western-blot方法的验证。结论溃疡性结肠炎患者肠黏膜存在vimentin降解片段蛋白水平的高表达,这种改变可能在在溃疡性结肠炎的发病机制中起重要作用。
Background The incidence of ulcerative colitis has been increasing year by year, but the pathogenesis is unknown. Many gene-based studies can not completely elucidate the changes of protein levels. Differential proteomics is a new method to study the pathogenesis of ulcerative colitis at the protein level. Objective To explore the pathogenesis of ulcerative colitis by proteomics. Methods 12 cases of active ulcerative colitis and 12 cases of normal intestinal mucosa were selected as study objects, 2-D electrophoresis was performed to extract the tissue proteins, silver staining and gel analysis were used to identify the differences, and mass spectrometry was used to identify the proteins. Western blotting was used to verify the difference point. Results Differential proteomics identified a total of 30 proteins (16 up-regulated and 14 down-regulated). The vimentin was successfully selected and verified by Western-blot. Conclusion There is a high expression of vimentin degrading fragment protein in intestinal mucosa of patients with ulcerative colitis. This change may play an important role in the pathogenesis of ulcerative colitis.