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克山病及化学药品中毒病人的红血细胞中常有一种小粒及小体。它对苏木精不染色,故称之为折射小粒及折射小体。其结构和所在是否与Heinz氏小体相似,我们作以下的观察。材料及方法取克山病人及中毒患者的血液作血膜片,甲醇固定,伊红——苏木精染色,水洗干燥后加松脂及盖片,作光学显微镜及3D聚光器检查。电子显微镜检查分为悬浮标本及超薄切片,悬浮标本先将红血细胞溶血,将其分散,用1％锇酸(pH 7.2-7.4)固定。超
Keshan disease and chemical poisoning patients often have small particles and small bodies in their red blood cells. It does not stain hematoxylin, so it is called refraction granules and refraction bodies. Its structure and location are similar to those of Heinz’s corpuscles, and we make the following observations. Materials and Methods Blood samples were taken from Keshan patients and patients with poisoned blood, fixed with methanol, stained with eosin-hematoxylin, washed with water and dried, then added with turpentine and coverslips for optical microscopy and 3D concentrator inspection. Electron microscopy is divided into suspension specimens and ultra-thin sections. The suspended specimens first hemolyze the red blood cells, disperse them, and fix them with 1% citric acid (pH 7.2-7.4). ultra