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目的:探讨穿山龙总皂苷对反复脑缺血再灌注小鼠学习记忆能力的影响及其作用机制。方法:昆明种小鼠70只随机分成5组,分别为假手术组,模型组,穿山龙总皂苷高、中、低剂量组(100、50、25 mg·kg-1·d-1),灌胃给药连续15 d。给药12 d采用双侧颈总动脉夹闭,建立反复脑缺血再灌注致小鼠学习记忆障碍模型,术后24 h和48 h通过跳台、Y型电迷宫实验,观察穿山龙总皂苷对反复脑缺血再灌注小鼠学习记忆能力的影响,同时对脑组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)、一氧化氮(NO)含量进行检测。结果:与模型组比较,穿山龙总皂苷可明显改善反复脑缺血再灌注小鼠的学习记忆能力(P<0.05或P<0.01),增强脑组织中SOD活性(P<0.05或P<0.01),降低MDA和NO含量(P<0.05或P<0.01)。结论:穿山龙总皂苷对反复脑缺血再灌注损伤小鼠的学习记忆功能具有改善作用,其机制可能与增强脑组织中SOD活力,降低MDA和NO含量有关。
Objective: To investigate the effect and its mechanism of Scutellaria total saponin on learning and memory in mice after repeated cerebral ischemia and reperfusion. Methods: Seventy Kunming mice were randomly divided into 5 groups: sham-operated group, model group, high, medium and low doses of Asplenium argenosides (100, 50, 25 mg · kg -1 · d -1) Stomach administration for 15 consecutive days. Administration of bilateral common carotid arteries for 12 days was performed to establish a model of learning and memory impairment induced by repeated cerebral ischemia and reperfusion in rats. After 24 and 48 hours of operation, At the same time, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) and nitric oxide (NO) in brain were detected. Results: Compared with the model group, total saponins of Radix Scutellariae could significantly improve the ability of learning and memory (P <0.05 or P <0.01) and enhance the activity of SOD in brain tissue of mice with repeated cerebral ischemia / reperfusion (P <0.05 or P <0.01) , Reduce the content of MDA and NO (P <0.05 or P <0.01). CONCLUSION: PNS can improve learning and memory in mice with repeated cerebral ischemia-reperfusion. Its mechanism may be related to the enhancement of SOD activity and decrease of MDA and NO in brain tissue.