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目的:了解广西地区丙型肝炎病毒(hepatitis C virus,HCV)基因型的分布特征,探讨HCV基因型与民族、性别、年龄、感染途径及HCV RNA载量的关系.方法:收集广西地区137例HCV RNA阳性患者的血清样本,采用逆转录巢式PCR(RT nested-PCR)法扩增HCV NS5B区段,对PCR终产物纯化测序后与Genbank中的标准株全基因序列比对,共同构建HCV NS5B区段系统进化树.结果:广西地区HCV基因型主要为1b型(58.4%),其次是6a型(12.4%),3b型(10.2%),1a型(7.3%),2a型(7.3%),3a型(4.4%).分析表明,HCV基因型与民族、性别无明显相关性(P>0.05),而与年龄、感染途径及HCV-RNA载量有一定相关性(P<0.05).结论:构建HCV NS5B区段系统进化树能得到准确的HCV基因型和亚型;广西地区至少存在7种HCV基因亚型,以1b型为主,6a型和3b型次之;较国内早期的研究报道,1b型的流行率似乎有下降趋势,而6a型的流行率似乎逐步上升;3型与6a型多见于有静脉药瘾史的年轻患者;随着感染途径的变迁,HCV基因型的分布也将发生改变.
Objective: To investigate the distribution of hepatitis C virus (HCV) genotypes in Guangxi and to explore the relationship between HCV genotype and ethnicity, sex, age, infection route and HCV RNA load.Methods: 137 cases of Guangxi HCV RNA positive patients serum samples were amplified by reverse transcription nested-PCR (RT-nested-PCR) HCV NS5B segment of the PCR product was purified and sequenced with GenBank standard sequence comparison of the complete genome to construct HCV NS5B segment phylogenetic tree.Results: The genotypes of HCV in Guangxi were 1b (58.4%), followed by 6a (10.2%), 1a (7.3%) and 2a %), And type 3a (4.4%). The analysis showed that HCV genotype had no significant correlation with ethnicity and gender (P> 0.05), but had some correlation with age, infection route and HCV- ) Conclusion: The construction of HCV NS5B segment phylogenetic tree can get accurate HCV genotypes and subtypes; there are at least seven HCV gene subtypes in Guangxi region, with type 1b predominating followed by type 6a and 3b; Early studies reported that the prevalence of type 1b seems to be declining, while the prevalence of type 6a appears to be gradually increasing; type 3 and type 6a are more common in the history of intravenous drug addiction Younger patients; with the changing routes of infection, the distribution of HCV genotype will also change.