【摘 要】
：
The bottom-up strategy for proteome analysis typically employs a multistep sample preparation workflow that suffers from being time-consuming and sample loss or
【机 构】
：
CAS Key Laboratory of Separation Sciences for Analytical Chemistry,National Chromatographic R&A Cent
论文部分内容阅读
The bottom-up strategy for proteome analysis typically employs a multistep sample preparation workflow that suffers from being time-consuming and sample loss or contamination caused by the off-line manual operation.Herein,we developed a hollow fibre membrane(HFM)-aided fully automated sample treatment(FAST)method.Due to the confinement effects of HFMs and the immobilized enzymatic reactor,the proteome samples could be denatured,reduced,desalted and digested within 8-20 min via the one-stop service.This method also showed superiority in trace sample analysis.In one and half hours,we could identify about 1,600 protein groups for 500 HeLa cells as the starting materials,1.5-8 times more than those obtained by previously reported methods.Through the on-line combination of FAST with nano-liquid chromatography-electrospray ionization tandem mass spectrometry(nanoLC-ESI-MS/MS),we further established a fully integrated platform for label-free quantification of proteome with high reproducibility and precision.Collectively,FAST presented here represents a major advance in the high throughput sample treatment and quantitative analysis of proteomes.
其他文献
Stem cell therapy is an attractive approach for recovery from myocardial infarction (MI) but faces the challenges of rapid diffusion and poor survival after tra
Although hydrazine(N2H4)oxidation in an electrochemical environment has been of great interest for years,its intrinsic electron transfer kinetics remain uncerta
历代方志皆以通纪为常态,以断代为例外,“一方全史”和“一地百科全书”成为人们普遍接受的方志印象.从两轮修志经验看,首轮修志始于改革开放后百业待兴的时代,编修通纪志恰
Cardiac fibroblast (CF) differentiation into myofibroblasts is a crucial cause of cardiac fibrosis,which increases in the extracellular matrix (ECM) stiffness.T
通过对网络服务提供者进行界定,区分提供信息内容服务的网络服务提供者和提供技术服务的网络服务提供者,前者承担直接侵权责任,后者承担间接侵权责任和替代责任;进一步分析网络服务提供者直接侵权责任、间接侵权责任、替代责任的责任认定和侵权责任构成要件,以此来作为证明责任分配的基础。由此认为:第一,直接侵权责任构成要件包括行为、过错、损害和因果关系四要件,网络服务提供者仅就“合法来源”承担证明责任。第二,间接侵权责任构成要件包括行为、过错、损害和因果关系四要件,网络服务提供者就“过错”要件承担证明责任。第三,替代侵权
Geminivirus infection triggers both transcriptional and posttranscriptional RNA silencing (RNA interference, RNAi)antiviral mechanisms.Much is known about the g
方志小序,其体源于先秦两汉,其成始于两宋.宋代,方志小序深受汉晋史书“论赞”“序例”影响,以释背景、述文旨、概大意、明义例为正宗.明清相承,民国重发明大要,贯通整体,彰
Oryza rufipogon Griff.is a wild progenitor of the Asian cultivated rice Oryza sativa.To better understand the genomic diversity of the wild rice,high-quality re
目的探究艾司西酞普兰与文拉法辛治疗焦虑症患者的临床效果。方法回顾性分析2017年3月至2019年6月于本院收治的98例焦虑症患者的临床资料,以治疗方案不同分为艾司西酞普兰组(n=33)、文拉法辛组(n=33)和联合组(n=32)。艾司西酞普兰组给予常规治疗+艾司西酞普兰治疗,文拉法辛组给予常规治疗+文拉法辛治疗,联合组给予常规治疗+艾司西酞普兰+文拉法辛治疗,比较3组汉密尔顿焦虑量表(HAMA)评分与不良反应发生情况。结果治疗后,3组HAMA评分均低于治疗前,且联合组HAMA评分低于艾司西酞普兰组、文拉法
Cenpj is a centrosomal protein located at the centrosomes and the base of cilia,it plays essential roles in regulating neurogenesis and cerebral cortex developm