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目的通过分析过敏孕妇脐血单核细胞来源树突状细胞(Dendritic cells,DCs)分泌细胞因子水平与正常孕妇来源DCs的差异,了解过敏来源树突状细胞功能的特点,为过敏性疾病的细胞学研究奠定基础,并为防治过敏性疾病寻找最佳时期。方法分离过敏及正常孕妇脐血内单核细胞,在GM-CSF及IL-4的作用下诱导生成未成熟DCs,在培养的第7天加入LPS(1μg/ml)诱导细胞成熟,阴性对照组仅加入细胞因子及培养基。于培养第9天收集培养上清,用ELISA法检测培养上清中IL-12p70及IL-10的分泌水平。结果过敏孕妇来源树突状细胞分泌细胞因子IL-12p70及IL-10的能力明显低于正常孕妇组。结论过敏孕妇来源树突状细胞可能存在功能上的缺陷,这可能是导致有过敏家庭史婴儿易患过敏性疾病的细胞学基础,孕期可能为防治过敏性疾病发生的最佳时期。
OBJECTIVE: To analyze the difference of DCs secreted by umbilical cord blood mononuclear cells derived from cord blood mononuclear cells and DCs derived from normal pregnant women to understand the function of dendritic cells from allergic pregnant women, Learn to lay the foundation for research, and to find the best period for the prevention and treatment of allergic diseases. Methods Umbilical cord blood mononuclear cells from allergic and normal pregnant women were isolated and induced to produce immature DCs under the action of GM-CSF and IL-4. LPS (1μg / ml) was added to induce cell maturation on the 7th day of culture. The negative control group Only add cytokines and media. The culture supernatant was collected on the 9th day of culture, and the secretion level of IL-12p70 and IL-10 in the culture supernatant was detected by ELISA. Results The ability of dendritic cells derived from allergic pregnant women to secrete cytokines IL-12p70 and IL-10 was significantly lower than that of normal pregnant women. Conclusions There may be functional defects in dendritic cells from allergic pregnant women. This may be the cytological basis for susceptible allergic diseases in infants with family history of allergy. Pregnancy may be the best period to prevent and cure allergic diseases.