论文部分内容阅读
建立了适于种子DNA分析的快速简便的PCR方法。水稻、小麦和玉米的半粒种子用提取缓冲液处理,产生的提取液可用于PCR和RAPD分析。水稻丰粒种子的DNA和来自叶片的DNA用专一的PCR引物扩增后可以产生同样的PCR产物。含有胚的另半粒种子可以正常发芽。将半粒种子的PCR分析方法用于水稻白叶枯病抗性基因型的鉴定,证明是植物育种和遗传学研究中一种非常实用的方法。
A quick and easy PCR method for seed DNA analysis was established. Half of the seeds of rice, wheat and corn are treated with extraction buffer and the resulting extract is used for PCR and RAPD analysis. The same PCR product can be generated after amplification of the rice seed with abundant DNA from the leaves and DNA from the leaves using a specific PCR primer. The other half seeds containing embryos germinate normally. The method of semi-seed PCR analysis for the identification of rice bacterial blight resistance genotypes proved to be a very practical method in plant breeding and genetics research.