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目的优化建立水产品中氧化三甲胺(trimethylamine-N-oxide,TMAO)离子色谱法测定的前处理方法,并对青岛地区常见水产品的氧化三甲胺含量进行测定。方法考察了三氯乙酸(TCA)法、Tris-TCA法、Tris-乙腈法三种常用的前处理方法对离子色谱测定结果的影响并进行了优化,收集了青岛常见水产中具有代表性的品种,采用非抑制性离子色谱法对氧化三甲胺含量进行了测定。结果测定了头足类、硬骨鱼类、贝类和甲壳类等21种青岛地区常见水产品中的氧化三甲胺含量。其中,阿根廷鱿鱼TMAO含量最高,为8791.89 mg/kg;硬骨鱼TMAO含量范围为350~2300 mg/kg;甲壳类除了刀额新对虾以外,含量均在1700 mg/kg之上;贝类TMAO含量均在500mg/kg之下。结论所测海产品中,头足类、甲壳类的氧化三甲胺含量较高,贝类含量较低,大致呈现头足类>甲壳类>硬骨鱼类>贝类的趋势。
Objective To optimize the pretreatment method for the determination of trimethylamine-N-oxide (TMAO) in aquatic products by ion chromatography and determine the content of trimethylamine oxide in common aquatic products in Qingdao. Methods The effects of TCA, Tris-TCA and Tris-TCA methods on the results of ion chromatography were studied and optimized. The representative species of common aquatic plants in Qingdao were collected. , The content of trimethylamine oxide was determined by non-suppressed ion chromatography. Results The contents of trimethylamine oxide in 21 kinds of aquatic products in Qingdao, such as cephalopods, teleosts, shellfish and crustaceans, were determined. Among them, the content of TMAO in Argentine squid was the highest (8791.89 mg / kg); the content of TMAO in bony fish ranged from 350 mg / kg to 2300 mg / kg; the content of crustaceans was above 1700 mg / kg, All below 500 mg / kg. Conclusion In the tested products, the content of trimethoxide in cephalopods and crustaceans is higher and the content of shellfish is lower, which roughly shows the tendency of cephalopods> crustaceans> teleosts> shellfish.