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目的观察圆窗局部灌注泊洛沙姆407对耳蜗功能和结构的影响。方法 16只健康豚鼠右耳圆窗灌注100μl 20%泊洛沙姆407溶液作为实验组,左耳灌注生理盐水作为对照组,另取4只动物不予处理作为阴性对照组。于灌注前、后及灌注后第7、14、28及49 d 行听性脑干诱发电位(auditory brainstem response,ABR)检测,并于检测后分别处死4只动物,行耳蜗基底膜铺片并计数。结果泊洛沙姆407灌注后 ABR 阈值较灌注前均有所提高,但在49 d 时恢复至灌注前水平;耳蜗毛细胞计数显示泊洛沙姆407圆窗灌注后无明显毛细胞缺失。结论泊洛沙姆407圆窗灌注对 ABR阈值有暂时性影响,但未对耳蜗功能和结构起到持久性不可逆性损伤,符合中、内耳疾病的缓释给药治疗的条件。
Objective To observe the effects of circular window topical perfusion of poloxamer 407 on the function and structure of the cochlea. Methods Twenty-six healthy guinea pigs were infused with 100μl 20% poloxamer 407 solution into the right ear round window as control group. The left ear was infused with normal saline as the control group, and the other 4 animals were not treated as the negative control group. Auditory brainstem response (ABR) was measured at 7, 14, 28 and 49 days after perfusion, and 4 animals were sacrificed after the perfusion. Cochlear basal membrane was plated count. Results The ABR threshold of poloxamer 407 was higher than that before perfusion, but returned to pre-perfusion level at 49 days. Cochlear hair cell count showed no obvious hair loss after polosham 407 injection. Conclusion Polydam 407 round window perfusion has a temporary effect on the ABR threshold, but it does not cause permanent irreversible damage to the cochlear function and structure. It is in line with the conditions of slow-release drug delivery for middle and inner ear diseases.