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[Objective] The aim of this study was to introduce Phosphoenolpyruvate Carboxylase(PEPCase) gene into common wheat Linyou 145.[Method] With the material of common wheat Linyou 145,Phosphoenolpyruvate Carboxylase(PEPCase) gene was introduced into wheat embryo callus by the agrobacterium-mediated transformation system,and then analyzed through successive selection with selective medium containg gygromycin to detect the gene at the molecular level.[Result] The hyg-resistant plants were obtained,and GUS histochemical staining showed the leaf of resistant plants was stained dark blue.The target bands appeared in PCR analysis.[Conclusion] Phosphoenolpyruvate Carboxylase(PEPCase) gene has been primarily introduced into the recipient material.
[Objective] The aim of this study was to introduce Phosphoenolpyruvate Carboxylase (PEPCase) gene into common wheat Linyou 145. [Method] With the material of common wheat Linyou 145, Phosphoenolpyruvate Carboxylase (PEPCase) gene introduced into wheat embryo callus by the agrobacterium -mediated transformation system, and then analyzed through successive selection with selective medium containg gygromycin to detect the gene at the molecular level. [Result] The hyg-resistant plants were obtained, and GUS histochemical staining showed the leaf of resistant plants was stained dark blue The target bands were in PCR analysis. [Conclusion] Phosphoenolpyruvate carboxylase (PEPCase) gene has been incorporated into the recipient material.