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目的:检测高同型半胱氨酸血症(Hhcy)大鼠阴茎组织内一氧化氮合酶(NOS)和一氧化碳(CO)的含量并探讨其对阴茎勃起功能的影响。方法:取成年雄性Wistar大鼠20只,随机均分为2组:正常对照组和Hhcy组。Hhcy组给予3%高蛋氨酸饲料喂养,正常对照组给予普通饲料喂养,4周后取血,测定血清Hhcy含量;取阴茎海绵体组织、匀浆,测定NOS和CO的含量。结果:Hhcy组血清中同型半胱氨含量[(22.32±1.65)μmol/L]显著高于正常对照组[(4.90±1.73)μmol/L],阴茎组织中的NOS[(6.45±1.12)nmol/(g·min)]和CO[(10.60±0.92)μmol/L]含量明显低于正常对照组[(10.77±0.60)nmol/(g·min)和(13.36±0.44)μmol/L]。结论:给予4周高蛋氨酸饮食的Wistar大鼠能引起Hhcy。Hhcy大鼠阴茎海绵体组织中内源性NOS和CO降低。Hhcy是大鼠阴茎勃起功能障碍的独立危险因素。
Objective: To investigate the content of nitric oxide synthase (NOS) and carbon monoxide (CO) in the penile tissue of hyperhomocysteinemia (Hhcy) rats and to explore its effect on penile erectile function. Methods: Twenty adult male Wistar rats were randomly divided into 2 groups: normal control group and Hhcy group. The Hhcy group was fed with 3% high methionine diet. The normal control group was fed with normal feed. After 4 weeks, the blood was taken and the serum Hhcy level was measured. The corpus cavernosum tissue and homogenate were taken for determination of NOS and CO contents. Results: The serum levels of homocysteine in Hhcy group were significantly higher than those in normal control group ([(22.32 ± 1.65) μmol / L, [4.90 ± 1.73] μmol / L] / (g · min)] and CO [(10.60 ± 0.92) μmol / L] were significantly lower than those in the normal control group [(10.77 ± 0.60) nmol / (g · min) and (13.36 ± 0.44) μmol / L], respectively. Conclusion: Wistar rats given a 4-week high methionine diet elicited Hhcy. Endogenous NOS and CO decreased in Hcy male rat corpus cavernosum. Hhcy is an independent risk factor for erectile dysfunction in rats.