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目的对3起不明原因的聚集性急性呼吸系统疾病暴发疫情的标本进行多重巢式PCR检测,及时筛查SARS病毒、高致病性人禽流感H5N1亚型病毒并检测其他亚型流感病毒、副流感病毒、支原体、肺炎衣原体等病原体,探讨该方法在聚集性急性呼吸系统疾病调查中的应用意义。方法采用多重巢式PCR方法对3起疫情的咽拭标本进行可引起急性呼吸系统疾病的23种病原体进行筛查。结果医院甲不明原因肺炎聚集性病例9例,对9例咽拭标本进行筛查,肺炎衣原体感染6例;学校乙不明原因肺炎聚集性病例69例,对13份咽拭标本进行检测,B型流感嗜血杆菌(HIB)感染7例;学校丙不明原因肺炎聚集性病例12例,对12份咽拭标本进行检测,HIB感染5例。结论多重巢式PCR方法可快速有效地筛查引起聚集性急性呼吸系统疾病暴发疫情的病原体,为有效遏制疫情及进一步处理提供线索。
OBJECTIVE: To carry out multiplex nested-PCR (PCR) detection on three unrelated samples of outbreaks of conglomerative acute respiratory diseases and to screen for SARS virus, H5N1 highly virulent avian influenza virus in a timely manner and to detect other subtype influenza viruses. Influenza virus, mycoplasma, Chlamydia pneumoniae and other pathogens, to explore the significance of this method in the investigation of aggregated acute respiratory diseases. Methods Multiple nested PCR (PCR) was used to screen the throat swab specimens of three outbreaks for 23 pathogens causing acute respiratory diseases. Results Nine patients with unexplained pneumonia in the hospital were screened for nine throat swabs and six were infected with Chlamydia pneumoniae. Sixty-nine patients with unexplained pneumonia were detected in the hospital. Thirteen throat swab specimens were examined. Type B Haemophilus influenzae (HIB) infection in 7 cases; school C unexplained pneumonia cases of aggregation in 12 cases, 12 swallowing specimens were tested, HIB infection in 5 cases. Conclusion The multiplex nested-PCR method can rapidly and effectively screen the pathogens that cause the outbreak of conglomerative acute respiratory diseases and provide clues for effectively stopping the outbreak and further treatment.