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为观察乙型肝炎和丙型肝炎双重感染的尸体肝组织中两种病毒核酸及抗原的分布,对15例HBV与HCV双重感染的尸检肝组织用免疫组织化学法检测HBsAg、HBcAg和HCAg.Digoxigenin标记的探针原位杂交法分别检测HBV DNA和HCV RNA。结果:HBsAg、HBcAg、HBV DNA、HCAg和HCV RNA的阳性数分别为12/15(80.0％)、10/15(66.7％)、9/15(60.0％)、11/15(73.3％)和12/15(80.0％)。HCAg和HCV RNA均可见于受染肝细胞胞浆或胞核内。材料多取自长期HBV感染后肝硬化的患者,多次输注血制品可能导致感染丙肝。结论:多数HBV与HCV双重感染者肝内同时存在两种病毒的抗原及核酸。
In order to observe the distribution of two kinds of virus nucleic acid and antigen in liver tissues of hepatitis B and hepatitis C infected double infection, immunohistochemical detection of HBsAg, HBcAg and HCAg in 15 cases of HBV and HCV double infection autopsy liver were performed.Digoxigenin Labeled probe in situ hybridization was used to detect HBV DNA and HCV RNA, respectively. RESULTS: The positive numbers of HBsAg, HBcAg, HBV DNA, HCAg and HCV RNA were 12/15 (80.0%), 10/15 (66.7%), 9/15 (60.0%), 11/15 12/15 (80.0%). Both HCAg and HCV RNA are found in the cytoplasm or nucleus of infected hepatocytes. Materials and more from long-term HBV infection in patients with cirrhosis, multiple blood transfusions may lead to hepatitis C. Conclusion: The majority of both HBV and HCV co-infected with two virus antigens and nucleic acids in the liver.