论文部分内容阅读
目的比较新型铂类化合物LLC-0601与舒铂、顺铂体外对人胚胎肝细胞(L-02)和人肾小管上皮细胞(HK-2)的毒性。方法采用不同浓度的LLC-0601、顺铂、舒铂分别与L-02细胞和HK-2细胞作用48 h后,四甲基偶氮唑盐(MTT)法检测并计算细胞增殖抑制率,计算IC50;取细胞培养液全自动生化分析仪检测乳酸脱氢酶(LDH)漏出量;显微镜下观察细胞形态改变。结果 LLC-0601、顺铂、舒铂作用两株细胞48 h后,同等剂量下LLC-0601对L-02细胞和HK-2细胞的抑制率都是最低,舒铂次之,顺铂最强,组间比较差异有统计学意义(P<0.05);检测两株细胞培养液中LDH含量,LLC-0601在较高剂量下才出现LDH升高,而顺铂和舒铂在较低剂量下LDH既有明显变化,组间比较差异有统计学意义(P<0.05),其中顺铂又强于舒铂(P<0.05)。3个铂类化合物细胞形态改变均表现随给药浓度增加,出现细胞核空泡样改变,细胞膜不完整,继续增加给药浓度后细胞死亡。结论 LLC-0601体外对L-02和HK-2细胞的毒性明显低于顺铂、舒铂,是一个有开发前景的新化合物。
OBJECTIVE: To compare the toxicity of cisplatin and LLC-0601 against human embryonic liver cells (L-02) and human renal tubular epithelial cells (HK-2) in vitro. Methods The inhibitory rates of LLC-0601, cisplatin and cisplatin were respectively detected by MTT assay after treated with L-02 cells and HK-2 cells for 48 h. IC50; cell culture fluid automatic biochemical analyzer to detect lactate dehydrogenase (LDH) leakage; observed under a microscope cell morphology. Results After treated with LLC-0601, cisplatin and cisplatin for 48 h, the inhibitory rates of LLC-0601 on L-02 cells and HK-2 cells at the same dose were the lowest, followed by cisplatin and cisplatin (P <0.05). Detection of LDH content in two cell culture medium, LLC-0601 LDH increased at higher doses, while cisplatin and cisplatin at lower doses LDH both had significant changes, the difference between the two groups was statistically significant (P <0.05), which was stronger than cisplatin and cisplatin (P <0.05). The morphological changes of the three platinum-based compounds showed an increase in drug concentration, a change in the vacuolar nuclei, incomplete cell membrane, and a continued increase in cell death after administration of the drug. Conclusion The toxicity of LLC-0601 to L-02 and HK-2 cells in vitro is significantly lower than that of cisplatin and cisplatin, which is a promising new compound.