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高压静电场预处理大豆种子不但提高了大豆种子抗低温吸胀冷害的能力,而且影响到子叶期到第一真叶期幼苗的抗低温胁迫的能力。处理组幼苗在2-4°C低温胁迫下比对照组有较高的过氧化物酶(POD),过氧化氢酶(CAT)及超氧化物歧化酶(SOD),淀粉酶(AML)的活力,预处理还降低了幼苗子叶中丙二醛(MDA)的积累量和电解质渗漏。从可溶性蛋白质含量的变化也显示出处理组幼苗较对照组幼苗抗冷害能力有了提高。本文从自由基伤害学说的角度作了探讨
The pretreatment of soybean seeds with high voltage electrostatic field not only enhanced the ability of soybean seeds to resist chilling injury of low temperature, but also affected the ability of low temperature stress resistance of seedlings in the first leaf stage. Compared with the control group, the seedling of treated group had higher activities of POD, CAT and SOD, AML than the control at low temperature of 2-4 ° C Vitality, pretreatment also reduced malondialdehyde (MDA) accumulation and electrolyte leakage in seedling cotyledons. The change of soluble protein content also showed that the seedlings of treated group had higher ability of resisting chilling injury than the control group. This article discusses the theory of free radical damage