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目的建立老鸦瓣芽茎愈伤组织诱导及丛生芽增殖体系。方法以老鸦瓣冷藏芯芽产生的芽茎为外植体,MS为基本培养基,考察不同质量浓度6-BA、NAA对愈伤诱导、分化及丛生芽增殖的影响。结果芽茎诱导愈伤的最佳培养基为MS+6-BA 0.5 mg/L+NAA 2.0 mg/L,愈伤诱导率78.54%,诱导出的愈伤组织在原培养基上继代培养增殖后即可进行芽分化;愈伤分化不定芽最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L,芽诱导率为66.21%;丛生芽增殖培养基为MS+6-BA 0.5mg/L+NAA 0.2 mg/L,增殖系数2.48。结论筛选出芽茎诱导愈伤、分化不定芽及丛生芽增殖的培养基,初步建立了老鸦瓣芽茎组织培养体系。
OBJECTIVE To establish the callus induction and shoot proliferation of Laogua bud bud. Methods The shoots stems from the core - shoots of Populus tomentosa used as explants and MS as the basic medium. The effects of different concentrations of 6 - BA and NAA on callus induction, differentiation and proliferation of clustered buds were investigated. Results The best medium for callus induction was MS + 6-BA 0.5 mg / L + NAA 2.0 mg / L and callus induction rate 78.54%. The induced callus was subcultured on the original medium The optimum culture medium for adventitious buds was MS + 6-BA 2.0 mg / L + NAA 0.2 mg / L, bud induction rate was 66.21%. The proliferation of MSCs was 6 + BA 0.5mg / L + NAA 0.2 mg / L, multiplication coefficient of 2.48. Conclusion The medium of callus induction, differentiation of adventitious shoots and proliferation of clustered buds were screened out.