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用γ辐射体同位素5—~(125)碘—2′—脱氧尿嘧啶核苷(~(125)IudR)参入分裂的肿瘤细胞的DNA中,测定这种靶细胞的~(125)IudR释放,是肿瘤免疫检验的一种较好的方法。我们研究了用~(125)IudR标记S—180、HAC、LAC、EAC、Eca109、D—6和Hella细胞的标记条件,并进行了正常和带瘤小鼠的脾淋巴细胞和腹腔巨噬细胞细胞毒试验,试验表明:靶细胞的制备对结果有重要影响,因此必须选择合适的试验条件,如肿瘤细胞的种类和来源,细胞浓度,~(125)IudR用量和标记时间等。本文讨论了这些问题,并介绍了试验方法。
The (125) IudR release of this target cell was measured using a gamma-irradiation isotope 5-125 iodine-2′-deoxyuridine (125) IudR into the DNA of the dividing tumor cells. It is a better method for tumor immunoassay. We studied the labeling conditions of S-180, HAC, LAC, EAC, Eca109, D-6, and Hella cells with ~(125)IudR, and performed splenic lymphocytes and peritoneal macrophages in normal and tumor-bearing mice. Cytotoxicity tests have shown that the preparation of target cells has an important influence on the results. Therefore, suitable experimental conditions must be selected, such as the type and source of tumor cells, cell concentration, ~(125) IudR dosage, and labeling time. This article discusses these issues and introduces test methods.