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选用Wistar雌性大鼠40只,分设正常、神经损伤(钳伤右侧坐骨神经干)、损伤对照组.运用免疫组化PAP方法对神经于内的S—100蛋白检测.染色结果经形态学观察及电子计算机图像处理系统分析结果表明:坐骨神经干内S—100蛋白含量与损伤后神经再生时间有密切关系,并观察到损伤后第5、7天,损伤神经侧S—100轴浆转运现象,推测S—100可能参于了周围神经系统再生微环境的变化,从而影响损伤神经元的存活和神经纤维的再生.
Forty Wistar female rats were selected and divided into normal and injured nerves (the right sciatic nerve of calf injury), and the control group was sacrificed.The immunohistochemical PAP method was used to detect the neuronal S-100 protein.The results of staining were observed by morphological observation and Computer image processing system analysis results show that the content of S-100 protein in sciatic nerve trunk is closely related to the time of nerve regeneration after injury, and the phenomenon of axonal S-100 axonal transport is observed on the 5th and 7th day after injury S-100 may participate in the changes of the microenvironment of the peripheral nervous system regeneration, thus affecting the survival of injured neurons and the regeneration of nerve fibers.