烟草低头黑的研究-1.诊断与接种方法

来源 :山东农业科学 | 被引量 : 0次 | 上传用户:Fzz
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烟草低头黑系山东烟区的一种严重病害。经过接种与观察,对其症状的发展作了较详细的记载,并和有关烟草病害的症状作了比较,以利区别。经多次分离、接种和培养,证明致病病菌为Colletotrichum sp.借单孢子分离发现病原所具曲梭形和长椭圆形两种类型的孢子,均能萌发,而且每一类型的孢子均能同时再产生两种形状的孢子。从已进行的孢子悬浮液喷射接种、移栽前土壤接种、移栽后土壤接种、插埋接种、补绽嫁接接种与伤口接种等六种接种法试验中,可以看出每一接种方法的发病率分别为100%、95.83%、100%、100%、75%和100%。从发病率、死亡率、接近自然接种情况,降低或然率和工作方便等条件全面考虑,以插埋法比较适用。 Tobacco bow black Department of Shandong tobacco smoke a serious disease. After vaccination and observation, the development of their symptoms made a more detailed record, and the symptoms of tobacco disease were compared to facilitate the difference. After many times of isolation, inoculation and culture, the pathogen was proved to be Colletotrichum sp. By single spore separation, both pathogenic and long oval spores germinated, and each type of spore At the same time, two kinds of spores are produced. From the vaccination of the spore suspension, the inoculation of soil before transplanting, the inoculation of soil after transplanting, the inoculation of inoculation, the grafting inoculation and the wound inoculation, we can see that the incidence of each inoculation method Rates were 100%, 95.83%, 100%, 100%, 75% and 100% respectively. From the morbidity, mortality, close to the natural inoculation, to reduce the probability and convenience of work and other conditions fully considered, burial method is more applicable.
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