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利用吸收光谱和荧光光谱方法，研究了吖啶橙（ＡＯ）与质粒ＤＮＡ水溶液、以及含胶束介质的吖啶橙与质粒ＤＮＡ溶液体系的相互结合作用及减色效应。结果表明：吖啶橙对质粒ＤＮＡ的吸收光谱有减色效应；含十二烷基硫酸钠（ＳＤＳ）的ＡＯ水溶液体系中，随着ＳＤＳ浓度的增加，其光谱结果表现为由凝聚态向单体的转化。而在含十二烷基硫酸钠（ＳＤＳ）的ＡＯ与质粒ＤＮＡ溶液体系中，吖啶橙凝聚态随ＳＤＳ浓度的增加，对ＡＯ与质粒ＤＮＡ相互结合产生协同的减色效应，使质粒ＤＮＡ空间结构发生缩拢。进一步采用电泳法研究了ＡＯ凝聚态可能对质粒ＤＮＡ构象的影响，结果表明：在ＡＯ与质粒ＤＮＡ溶液体系中，ＡＯ浓度的增加对质粒ＤＮＡ构象未产生影响；而在含有ＳＤＳ的ＡＯ与质粒ＤＮＡ的溶液体系中，由于ＳＤＳ对ＡＯ凝聚态的解聚作用，以及ＳＤＳ对质粒ＤＮＡ减色效应的协同作用，使得质粒ＤＮＡ的构象发生变化，诱导质粒ＤＮＡ形成超螺旋构象
The interaction between acridine orange (AO) and plasmid DNA aqueous solution and acridine orange and plasmid DNA solution system containing the micellar medium and the effect of reducing color were studied by absorption and fluorescence spectroscopy. The results showed that acridine orange had a hypochromic effect on the absorption spectra of plasmid DNA. With the increase of SDS concentration, the results of the aqueous solution of sodium dodecyl sulfate (SDS) Body transformation. In AO and plasmid DNA solution system containing sodium dodecyl sulfate (SDS), acridine orange condensed state with the increase of SDS concentration, the combination of AO and plasmid DNA produce a synergistic reduction effect, the plasmid DNA space The structure shrinks. The effect of AO aggregation on the conformation of plasmid DNA was further studied by electrophoresis. The results showed that the increase of AO concentration did not affect the conformation of plasmid DNA in AO and plasmid DNA solution systems. However, Solution system, the disaggregation of the condensed state of the AO by the SDS and the synergistic effect of the SDS on the subtractive effect of the plasmid DNA change the conformation of the plasmid DNA to induce the supercoiled conformation of the plasmid DNA