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目的了解广西肠道病毒的感染情况,为制定预防和控制肠道病毒感染策略提供依据。方法采用实时荧光定量PCR方法,对广西253例疑似肠道病毒感染者咽拭子、疱疹液和粪便标本共505份进行肠道病毒、肠道病毒71型(EV71)和柯萨奇病毒A16型(CoxA16)核酸检测。结果共检出290份肠道病毒核酸阳性,阳性率为57.43%,其中EV71核酸阳性105份,阳性率为20.79%,CoxA16核酸阳性143份,阳性率为28.32%。结论 505份标本中CoxA16核酸的阳性率高于EV71;疱疹液标本的阳性检出率高于肛拭子、咽拭子、粪便标本的阳性检出率;CoxA16和EV71是引起儿童手足口病的主要病原体,要加强对CoxA16和EV71的的鉴别诊断。开展手足口病流行病学和病原学研究,将有助于提出更好的预防和控制措施。
Objective To understand the prevalence of enterovirus infection in Guangxi and provide evidence for the prevention and control of enterovirus infection. Methods The real-time PCR method was used to detect the enterovirus, enterovirus 71 (EV71) and coxsackievirus A16 type in 505 samples of throat swab, herpes fluid and stool specimens of 253 suspected cases of enterovirus infection in Guangxi. (CoxA16) nucleic acid test. Results A total of 290 positive samples of enterovirus nucleic acid were detected, with a positive rate of 57.43%, of which 105 positive for EV71 were positive, 20.79% for positive EV71, and 143 positive for CoxA16. The positive rate was 28.32%. Conclusion The positive rate of CoxA16 in 505 specimens was higher than that in EV71. The positive rate of herpes liquid specimens was higher than that of anal swabs, throat swabs and stool specimens. CoxA16 and EV71 were the cause of HFMD in children The main pathogens, to strengthen the differential diagnosis of CoxA16 and EV71. Developing HFD epidemiology and etiology will help to propose better prevention and control measures.