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本文针对饮用水系统中营养和抗生素水平对质粒抗生素抗性的适应度代价进行了研究.选取了p ACYC184、RP4和PBR322这3种不同的抗性质粒,发现在不同的营养水平下,抗性质粒的适应度代价具有普遍性.而且,营养水平越低,其适应度代价表现得越明显.另外,痕量浓度的抗生素也会影响抗性质粒的适应度代价.本研究中,小于50%MIC的抗生素浓度对野生菌的损伤或抑制作用不足以抗衡抗性质粒的适应度代价,质粒难以维持.因而,推断在饮用水系统(贫营养和ng·L-1水平的抗生素)中,质粒抗生素抗性的适应度代价较大,质粒不易维持,在饮用水系统中传播和扩散的风险较小.
In this paper, the adaptability of antibiotic and antibiotic levels in drinking water systems was studied, and three different resistant plasmids, pACYC184, RP4 and PBR322, were selected and tested for their resistance to different nutritional levels The fitness of plasmids is universal, and the lower the nutrition level, the more obvious its fitness cost is.In addition, trace concentrations of antibiotics also affect the fitness cost of resistant plasmids.In this study, less than 50% Therefore, it is inferred that in the drinking water system (antibiotic at the level of ng / L-1 at the level of oligotrophic bacteria), the plasmid DNA is inferred from the antibiotic concentration of MIC not to impair the fitness or the inhibitory effect on the wild type bacteria in order to counter the fitness cost of the resistant plasmid Antibiotic resistance is more costly to adapt, less stable plasmids, and less risk of spreading and spreading in drinking-water systems.