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目的 探讨骨调素 ( OPN)在阿霉素肾病模型中的表达和作用以及血管紧张素 ( Ang )受体拮抗剂对其表达的影响。方法 将实验动物随机分为正常对照组 ( C组 ) ,阿霉素肾病组 ( A组 )和厄贝沙坦治疗组 ( IR组 ) ,第2、 4周用免疫组化方法检测各组肾内 OPN和单核巨噬细胞抗原 ( ED1)的表达情况。结果 发现在第 2周和第 4周 ,A组和 IR组 OPN与 ED1表达水平均明显高于正常组 ( P<0 .0 5 ) ,而 IR组 OPN与 ED1表达水平均低于 A组 ( P<0 .0 5 )。在第 2周和第 4周 ,A组 OPN与 ED1的表达水平呈显著正相关 ( r=0 .92 2 ,P<0 .0 1;r=0 .80 0 ,P<0 .0 5 )。结论 OPN在阿霉素肾病这种微小病变模型中是一种重要的趋化因子 ,与肾小管间质单核巨噬细胞浸润密切相关 ,使用 Ang 受体拮抗剂可使该模型 OPN表达下调 ,从而减轻了炎性细胞浸润和肾损伤
Objective To investigate the expression and effect of osteopontin (OPN) in adriamycin nephropathy model and the effect of angiotensin (Ang) receptor antagonist on its expression. Methods The experimental animals were randomly divided into normal control group (group C), adriamycin nephropathy group (group A) and irbesartan treatment group (IR group). At the second and fourth weeks, Expression of OPN and monocyte-macrophage antigens (ED1). The results showed that the expression levels of OPN and ED1 in group A and group IR were significantly higher than those in normal group (P <0.05) at 2 weeks and 4 weeks, while those in group IR were lower than those in group A P <0. 05). At the second week and the fourth week, the expression of OPN in group A was positively correlated with ED1 (r = 0.92 2, P <0.01; r = 0.80 0, P <0.05) . Conclusion OPN is an important chemotactic factor in adolescent nephropathy model of adriamycin, which is closely related to the infiltration of tubulointerstitial mononuclear macrophages. The expression of OPN was down-regulated by Ang receptor antagonist, Thus reducing inflammatory cell infiltration and kidney damage