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本文对香菇(Lentinusedodes)单核菌丝9101(12)原生质体制备后的再生条件,进行了探索。结果表明,原生质体经过合0.6M.甘露醇的麦芽糖酵母粉培养基(MYG)液体培养5天,用双层培养法,在含0.6MMgSO4的高渗MYG平板上经再生,再生率达5×10-5。本文将Knowlton等于1984年创造的一种分离高频再生衍生株的方法首次运用至食用菌中,所得9101(12)再生株R3,再生率提高2~3倍,为香菇原生质体融合操作打下基础。
In this paper, the regeneration conditions of protoplasts of Lentinousodes monokaryon 9101 (12) were explored. The results showed that the protoplasts through the combined 0.6M. The mannitol maltose yeast culture medium (MYG) liquid was cultured for 5 days, and was regenerated on a hypertrophic MYG plate containing 0.6MMgSO4 by a double layer culture method at a regeneration rate of 5 × 10-5. In this paper, a method of separating high frequency regenerated derivative strains, which is created by Knowlton et al. In 1984, was first applied to edible fungi. The obtained 9101 (12) regenerated strain R3 improved the regeneration rate by 2 to 3 times, laying a foundation for the protoplast fusion of mushroom .