采用MPC和CVP富集培养基从马唐和狗尾草的根部分离到假单胞菌792株、欧文氏菌515株。通过对大肠杆菌的抗代谢毒性试验、对原宿主的萌发抑制试验、杀草试验以及对两种草坪草的安全性试验,筛选的S7菌株能100％抑制狗尾草的种子萌发,对供试草坪草不仅没有负影响,而且对高羊茅的种子萌发还有轻微的促生作用。尽管S7在狗尾草的芽后处理中校正防效只有56.7％,但分析认为以杂草DRB作微生物除草剂的目标菌株会更有前途。 Pseudomonas sp. 792 and Erwinia sp. 515 were isolated from the roots of Crabgrass and Setaria viridis using MPC and CVP enrichment media. Through the anti-metabolic toxicity test of Escherichia coli, the germination inhibition test, the herbicide killing test and the safety test of two kinds of turfgrass, the screening S7 strain can inhibit the germination of Setaria viridis, Not only does it have no negative effects, but also has a slight effect on the germination of tall fescue seeds. Although S7 was only 56.7% correct in post-processing of Setaria viridis, the analysis suggested that the target strain with weedy DRB as a microbial herbicide would be more promising.