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本文比较研究了经过太空诱变的卤虫(Artemia sinica)同地面对照组卤虫4对选择性扩增引物的AFLP指纹图谱,旨在探讨用AFLP技术进行诱变个体遗传差异检测的可行性.其遗传相似系数用Nei的计算方法进行计算,遗传距离则用Lynch的计算方法进行计算.实验共检测出384个位点,分子量在50~1 500bp之间,其中绝大多数片段为太空诱变组同地面对照组所共有,多态位点73个,占19.0%.遗传指纹图谱显示:经过太空诱变的群体与地面对照组相比,从整个基因组角度来看,其遗传差异并不明显,但是太空诱变组有2个个体的基因确实产生了较为显著的变异.研究结果表明:太空诱变能够不定向地促进基因的变异,是水产新品种选育的途径之一;AFLP技术不仅是各水产动物种质鉴别的有效手段,用于各种诱变个体的遗传差异研究也是适宜和可信的.
In this paper, AFLP fingerprinting of four selective pairs of Artemia sinica and ground-control Artemia sinica were compared in order to explore the feasibility of using AFLP technique to mutate genetic differences in individuals. The genetic similarity coefficient was calculated by Nei’s calculation method, and the genetic distance was calculated by Lynch’s calculation method.A total of 384 loci were detected with the molecular weight between 50 ~ 1500bp, most of which were space mutagenesis There were 73 polymorphic loci, accounting for 19.0% of the total, with 19.0% of the total polymorphic loci.Genetic fingerprinting indicated that the genetic variation of space-mutated groups was not obvious compared with the ground-based control group , But there were two distinct gene mutations in space mutagenesis group.The results show that space mutagenesis can promote gene mutation without direction, which is one of the ways to breed new aquatic species. AFLP technology not only Is an effective means to identify the germplasm of aquatic animals. It is also appropriate and credible to study the genetic differences among various mutagenized individuals.