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单纯用化学致癌剂二乙基亚硝胺（DENA）及氯化钆（gadolinium chloride.GC）填塞枯否细胞,同时对DENA所诱发的大鼠肝癌形成过程中的大体形态、光镜及电镜下的病理学改变进行了对比研究。结果显示:①诱癌过程中,GC+DENA组大鼠自然死亡率明显高于DENA组（P<0.05）;自诱癌第19周后,GC+DENA组大鼠肝癌表面结节明显大于DENA组;②光镜GC+DENA组大鼠肝癌前病变出现远较DENA组为早;③电镜下证实:GC+DENA组肝窦内枯否细胞（KC）胞浆内含有大量电子致密物或细胞空泡变性及坏死;DENA组KC保持结构基本完整;前组肝细胞除核呈高度不规则性、核仁明显外.胞浆损伤主要表现为粗面内质网空泡变,后组肝细胞显示核结构改变与前组相同.胞浆则以线粒体肿胀、基质内絮状物沉积为突出病变。上述结果提示.氯化钆填塞KC可在一定程度下加速致癌剂对肝细胞的毒性损伤和肝细胞的癌变过程。
The use of chemical carcinogen diethylnitrosamine (DENA) and gadolinium chloride (GC) to occlude Kupffer cells, and the gross morphology, light microscopy, and electron microscopy of rat liver cancer induced by DENA The pathological changes were compared. The results showed that:1 In the process of carcinogenesis, the natural mortality rate of rats in GC+DENA group was significantly higher than DENA group (P<0.05). After the 19th week of cancer induction, the surface nodule of liver cancer in GC+DENA group was significantly greater than DENA. Group; 2 Light microscope precancerous lesions in GC + DENA group were earlier than those in DENA group; 3 electron microscopy confirmed: GC + DENA group liver antrum Kupffer cells (KC) cytoplasm contains a large number of electron compacts or cells Vacuolar degeneration and necrosis; KNA in the DENA group maintained a substantially complete structure; the hepatocytes in the anterior group were highly irregular and nucleoli in appearance. Cytoplasmic injury was mainly characterized by rough endoplasmic reticulum vacuolization and hepatocytes in the posterior group. The change in nuclear structure was similar to that of the former group. The cytoplasm was bulging with mitochondria and floc deposition in the matrix as the prominent lesion. The above results suggest that cesium chloride filling KC can accelerate the toxicity of carcinogens on hepatocytes and carcinogenesis of hepatocytes to a certain extent.