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目的观察槲皮素(Quercetin,Que)和异鼠李素(Isorhamnetin,Iso)在体外对已受到Cu2+、Fe2+氧化修饰的人血浆高密度脂蛋白(highdensitylipoprotein,HDL)的作用。方法采用一次性密度法分离正常人血浆HDL,用Cu2+,Fe2+进行体外氧化修饰。抗氧化组在修饰后6h加Que或Iso(100μmol/L)作用不同时间(4,8和16h)。分别检测HDL中脂质过氧化物丙二醛(MDA)、维生素E(VitE)含量及超氧化物歧化酶(SOD)活性。结果100μmol/LQue和Iso作用4,8h后可分别明显抵制Fe2+-Ox-HDL,Cu2+-Ox-HDL中MDA生成(P<0.001)。Que和Iso作用8,16h后可分别提高Cu2+-Ox-HDL,Fe2+-Ox-HDL中SOD活性(P<0.001);明显延缓VitE含量降低(P<0.05)。结论Que和Iso均对已受到Cu2+,Fe2+氧化修饰的HDL具有明显的抑制作用,但对Cu2+,Fe2+氧化修饰的HDL,槲皮素、异鼠李素的作用存在差异。Que和Iso的作用机制可能与其抗自由基氧化有关,提示该类物质是一种良好的抗氧化剂,对As的防治有一定的运用价值。
Objective To investigate the effects of quercetin (Que) and isorhamnetin (Iso) on human plasma HDL (high density lipoprotein) oxidized by Cu2 + and Fe2 + in vitro. Methods The plasma HDL of normal people was separated by one-time density method. The oxidative modification was performed in vitro with Cu2 + and Fe2 +. Antioxidant groups were treated with Que or Iso (100μmol / L) at different time (4, 8 and 16h) after 6h modification. HDL lipid peroxidation malondialdehyde (MDA), vitamin E (VitE) content and superoxide dismutase (SOD) activity were detected. Results After treated with 100μmol / L Que and Iso for 4 and 8 hours, MDA production in Fe2 + -Ox-HDL and Cu2 + -Ox-HDL were significantly inhibited (P <0.001). The activities of SOD and SOD in Cu2 + -Ox-HDL and Fe2 + -Ox-HDL increased after 8 and 16 h of Que and Iso treatment, respectively (P <0.001). Conclusions Both Que and Iso have significant inhibitory effects on HDL oxidatively modified by Cu2 + and Fe2 +, but there are differences in the effects of Cu2 + and Fe2 + oxidatively modified HDL, quercetin and isorhamnetin. The mechanism of action of Que and Iso may be related to their anti-free radical oxidation, suggesting that these substances are good antioxidants and have certain value in the prevention and treatment of As.