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为克服目前肿瘤基因治疗中载体系统效率低下,缺乏靶向性的不足,研究构建了受体介导的靶向性非病毒型基因转移系统,并利用该系统将p21基因导入体外培养的人脑胶质瘤细胞,观察p21对肿瘤细胞生长的抑制效应.方法:根据受体配体特异性结合理论,鉴于在多种肿瘤细胞表面存在表皮生长因子受体(EGF-R)的过量表达,分别设计合成了针对EGF-R的16肽GE7配体寡肽以及流感病毒血凝素功能域20肽HA20做为内吞小体释放寡肽,将两者分别与多聚阳离子鱼精蛋白(protamine)共价连接,籍静电效应与DNA形成复合体颗粒,构成受体介导的靶向性载体系统.以此载体系统携带β-gal报道基因转染体外培养的人脑胶质瘤细胞U251,x-
In order to overcome the low efficiency and lack of targeting of the vector system in tumor gene therapy, a receptor-mediated targeting non-viral gene transfer system was constructed and the p21 gene was introduced into the human brain cultured in vitro To observe the inhibitory effect of p21 on the growth of tumor cells.Methods: According to the specific binding theory of receptor ligands, in view of the overexpression of epidermal growth factor receptor (EGF-R) on the surface of various tumor cells, The 16-mer GE7 ligand oligopeptide targeting EGF-R and the HA20 peptide of the influenza virus hemagglutinin domain 20 were designed and synthesized to release oligopeptides as endocytosomes, which were respectively combined with polycation protamine Covalently linked to the electrostatic effect and the formation of DNA composite particles, constitute a receptor-mediated targeting vector system carrier vector carrying β-gal reporter gene was transfected in vitro cultured human glioma cells U251, x -