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目的 :通过检测衰老大鼠肝组织细胞线粒体呼吸链氧化功能 ,探讨大鼠衰老时肝组织细胞线粒体呼吸链氧化功能的变化规律及 94 1方延缓衰老的作用机制。方法 :分别从 4月龄 (青年组 )、 2 4月龄 (老龄组 )和 94 1方组大鼠的肝组织分离出线粒体 ,并用双缩脲法测定单位组织的线粒体蛋白 ,用Clark氧电极极谱法分别测定线粒体呼吸链 3个氧化酶活性 :NADH氧化酶(NADHOX)、琥珀酸氧化酶 (SUCOX)和细胞色素氧化酶 (CYTOX)。用差光谱法 (联二亚硫酸钠还原减正铁氢化钾氧化的消光值 )获得细胞色素a、b、c和c1含量 (Cyta、Cytb、Cytc和Cytc1)。结果 :与青年组大鼠比较 ,老年组大鼠的肝组织细胞线粒体含量有一定下降 ,而 3个氧化酶活性却显著增加 ,同时细胞色素含量尤其是Cytb和Cytc显著升高。 94 1方组大鼠肝组织细胞线粒体上述指标皆显著高于青年组和老年组。结论 :衰老大鼠肝组织线粒体酶活性与青年组有显著不同 ,表明衰老过程中肝细胞通过某种反馈机制使残存完好的线粒体氧化功能代偿性增强 ;94 1方使这种作用进一步增强。目的 :通过检测衰老大鼠肝组织细胞线粒体呼吸链氧化功能 ,探讨大鼠衰老时肝组织细胞线粒体呼吸链氧化功能的变化规律及 94 1方延缓衰老的作用机制。方法 :分别从 4月龄 (青年组 )、 2 4月龄 (?
Objective : To detect the oxidative function of mitochondrial respiratory chain in hepatic tissue of aged rats, to explore the changes of oxidative function of mitochondrial respiratory chain in hepatocytes of aging rats and the mechanism of anti-aging effects. METHODS: Mitochondria were isolated from liver tissues of 4-month-old (youth group), 24-month-old (old age group), and 94-square-group rats, and mitochondrial protein was determined by biuret method using Clark oxygen electrode. The polarographic method was used to determine three oxidase activities in the mitochondrial respiratory chain: NADHOX, SUCOX, and CYTOX. The cytochrome a, b, c, and c1 contents (Cyta, Cytb, Cytc, and Cytc1) were obtained by differential spectroscopy (reduction of the potassium hydrogen hydride by the sodium hyposulfite). Results :Compared with the young group rats, the mitochondrial content of hepatic cells in the aged group had a certain decrease, while the three oxidase activities increased significantly. At the same time, the cytochrome content especially Cytb and Cytc increased significantly. The mitochondria of hepatic cells in the rats of the group 1 94 were significantly higher than those of the young group and the old group. Conclusion : The activity of mitochondrial enzymes in aging rats was significantly different from that of the young group, indicating that hepatocytes through some kind of feedback mechanism during the aging process compensated the intact mitochondrial oxidative function; 94 1 way to further enhance this effect. Objective : To detect the oxidative function of mitochondrial respiratory chain in hepatic tissue of aged rats, to explore the changes of oxidative function of mitochondrial respiratory chain in hepatocytes of aging rats and the mechanism of anti-aging effects. Methods: From 4 months of age (youth group), 24 months of age (?