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目的:制备能同时实现T_1-T_2双模式磁共振成像的Fe_3O_4-BSA~(Gd)纳米粒子造影剂,并对其造影性能和生物相容性进行评价,以提高磁共振造影剂对病变部位诊断的敏感性及特异性,克服单一阳性造影剂和阴性造影剂的局限性,实现疾病的精准诊断。方法:采用高温热解法制备出油溶性粒径约6 nm的超顺磁性Fe_3O-4纳米粒子,随后利用超声乳化方式在其表面修饰顺磁性牛血清白蛋白-钆复合物(BSA~(Gd)),同时赋予Fe_3O_4纳米粒子顺磁性、水溶性和良好的生物相容性。并对其形貌、水合粒径(hydrated diameter,HD)、饱和磁化强度、弛豫效率(relaxation efficiency,r)、体外T_1和T_2加权成像效果、细胞毒性进行了表征。结果:Fe_3O_4-BSA~(Gd)纳米颗粒径为10 nm,水合粒径约32 nm,分散均匀稳定。Fe_3O_4-BSA~(Gd)的饱和磁化强度为30.2 emu/g。纵向(r_1)及横向(r_2)弛豫率分别为6.30 m M~(-1)s~(-1)和287.08 m M~(-1)s~(-1)。体外双模式成像结果显示其具有显著的T_1-T_2成像效果,并呈现浓度依赖性。细胞毒性测试结果显示,Fe_3O_4-BSA~(Gd)的生物相容性较好。结论:所制备的Fe_3O_4-BSA~(Gd)纳米粒子的体外T_1和T_2造影效果优良,生物相容性极好,为下一步的体内成像奠定了基础。
OBJECTIVE: To prepare Fe_3O_4-BSA ~ (Gd) nanoparticle contrast medium capable of simultaneous realization of T 1 -T 2 dual-mode magnetic resonance imaging and to evaluate its imaging performance and biocompatibility to improve the diagnosis of lesions Sensitivity and specificity to overcome the limitations of a single positive contrast agent and negative contrast agent to achieve accurate diagnosis of the disease. Methods: The superparamagnetic Fe_3O-4 nanoparticles with oil-soluble particle size of about 6 nm were prepared by high temperature pyrolysis. Subsequently, the surface of paramagnetic bovine serum albumin-gadolinium complex (BSA ~ (Gd) )), While giving Fe_3O_4 nanoparticles paramagnetic, water-soluble and good biocompatibility. The morphology, hydrated diameter (HD), saturation magnetization, relaxation efficiency (r), T 1 and T 2 weighted imaging in vitro and cytotoxicity were characterized. Results: The diameter of Fe 3 O 4-BSA ~ (Gd) nanoparticle was 10 nm and the hydration size was about 32 nm. The saturation magnetization of Fe_3O_4-BSA ~ (Gd) is 30.2 emu / g. The relaxation rates in the longitudinal direction (r_1) and lateral direction (r_2) were 6.30 m M -1 s -1 and 287.08 m -1 s -1, respectively. In vitro bi-modal imaging showed significant T 1 -T 2 imaging results in a concentration-dependent manner. Cytotoxicity test results showed that the biocompatibility of Fe 3 O 4 -BSA ~ (Gd) is better. CONCLUSION: The prepared Fe_3O_4-BSA ~ (Gd) nanoparticles have excellent T_1 and T_2 imaging results and excellent biocompatibility, which lays the foundation for the next in vivo imaging.