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本文选用 ~aH-TdR 掺入法测定脾淋巴细胞的增殖,DPH 标记淋巴细胞膜,荧光偏振法测定脾淋巴细胞和骨髓细胞的荧光偏振度。结果:DBA 纯系健康对照小鼠脾淋巴细胞增殖高,萤光偏振度较大,脂质流动性小;L_(1210)腹水癌小鼠脾淋巴细胞增殖大大减少,荧光偏振度减小,膜脂质流动性增大。0.4g/kg 脾肾方分别给健康和 L_(1210)小鼠灌胃9天后,脾肾方可使脾淋巴细胞增殖升高,并能调节膜脂质流动性接近于健康小鼠。对骨髓细胞膜脂质流动性也有相同作用。初步揭示了脾肾方对免疫不平衡动物 T 细胞功能的调节作用和骨髓细胞脾淋巴细胞膜脂质流动性相关的机制。
In this study, ~aH-TdR incorporation method was used to measure the proliferation of splenic lymphocytes, DPH was used to label the lymphocyte membrane, and fluorescence polarization was used to determine the fluorescence polarization degree of splenic lymphocytes and bone marrow cells. RESULTS: The proliferation of spleen lymphocytes of DBA pure healthy control mice was high, the degree of fluorescence polarization was large, and the lipid fluidity was small. The proliferation of splenic lymphocytes in L_(1210) ascites carcinoma mice was greatly reduced, and the degree of fluorescence polarization was reduced. Lipid fluidity increases. After spleen and kidney of 0.4g/kg were given to healthy and L_(1210) mice for 9 days respectively, spleen and kidney can increase the proliferation of splenic lymphocytes and regulate membrane lipid fluidity similar to healthy mice. It also has the same effect on the lipid fluidity of bone marrow cell membranes. The mechanism of spleen-kidney’s regulation of T-cell function in immunocompromised animals and the mechanism of lipid fluidity of splenic lymphocyte membranes in bone marrow cells were initially revealed.