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目的 探讨阳离子脂质体(天然碱性脂SA)携带碱性成纤维细胞生长因子/绿色荧光蛋白(bFGF/GFP)基因在豚鼠耳蜗中的表达,以及对庆大霉素所致耳蜗损害的防治作用。方法 将36只豚鼠分为3组,预防组右耳园窗注入SA-bFGF/GFP复合物后次日肌肉注射庆大霉素150mg.Kg~(-1).d~(-1)8天,治疗组先用庆大霉素8d后次日右耳给药,对照组单用庆大霉素8d。分别于实验前后及处死前行听觉脑干诱发电位(ABR)测试。荧光显微镜下观察耳蜗GFP的表达;用耳蜗琥珀酸脱氢酶染色铺片,扫描电镜观察毛细胞的缺失情况。结果 荧光显微镜下见双侧耳蜗均有GFP表达。预防和治疗组处死前的双耳ABR阈值与对照组比较差异有显著意义(P<0.01,P<0.05),耳蜗内外毛细胞缺失数与对照组比较差异有显著意义(P<0.01,P<0.05)。结论 SA脂质体介导的bFGF/GFP基因单耳给药双侧耳蜗均有高效表达,并对庆大霉素所致的耳蜗损害有防治作用。
Objective To investigate the expression of basic fibroblast growth factor / green fluorescent protein (bFGF / GFP) in cochlea of guinea pig with cationic liposome (natural lipid SA) and the prevention and treatment of cochlear damage induced by gentamicin effect. Methods Thirty-six guinea pigs were divided into three groups. Gentamicin 150 mg · kg -1 was intramuscularly injected into SA-bFGF / GFP injection group , The treatment group first with gentamicin 8d the next day right ear administration, the control group alone gentamicin 8d. Auditory brainstem response (ABR) test was performed before and after the experiment and before sacrifice. The expression of GFP in the cochlea was observed under a fluorescence microscope. The cochlea was stained with succinate dehydrogenase and observed for the absence of hair cells by scanning electron microscopy. Results GFP expression was found in bilateral cochlea under fluorescence microscope. There were significant differences in ABR thresholds between two groups (P <0.01, P <0.05), and the number of hair cells missing in the prevention and treatment group compared with the control group (P <0.01, P < 0.05). Conclusions SA liposome-mediated bFGF / GFP gene is highly effective on both sides of cochlea, and it can prevent and treat cochlear damage caused by gentamicin.