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Objective.To investigate the frequency of p16a nd p15gene methylation in multiple myeloma (MM),and its relationship with bone marrow ce ll apoptosis and clinical outcome.Methods.Twenty-two patients with MM were stu died to detect p16and p15gene methylation.Methyla-tion-specific polymerase chain rea ction(MSP)was used to detect gene methylation,and terminal trans-ferase-mediated dUTP nick end-labeling(TUNEL)was used to detect cell apoptosis.Results.p16and /or p15gene methylatoin was d etected in 10of 22patients(45.4%).There were 3pa-tients with p16gene methylation,9p atients with p15gene methylation,a nd 2patients with both genes methyla-tion.The incidence of methylation o f p15gene was higher than that of p16g ene(P<0.05).The patients with p16and /or p15gene methylation had a delayed cell apoptosis,poor respon se to chemotherapy,and a short over-all survival(OS).Conclusion.The methylation of p16and /or p15gen e plays a key role in MM apoptosis path ogenesis.The patients with both p16and p15gene me thylation had a poor prognosis.
Objective.To investigate the frequency of p16a nd p15gene methylation in multiple myeloma (MM), and its relationship with bone marrow ll ll apoptosis and clinical outcome. Methods. Two-two patients with MM were stu died to detect p16 and p15gene methylation. Methyla- tion-specific polymerase chain rea ction (MSP) was used to detect gene methylation, and terminal trans-ferase-mediated dUTP nick end-labeling (TUNEL) was used to detect cell apoptosis. Results. p16 and / or p15gene methylatoin was d etected in 10 of 22 patients (45.4%). There were 3 pa-tients with p16 gene methylation, 9 p atients with p15 gene methylation, a nd 2 patients with both genes methyla- tion. The incidence of methylation of p15 gene was higher than that of p16 g ene (P <0.05) . The patients with p16 and / or p15 gene methylation had a delayed cell apoptosis, poor respon se to chemotherapy, and a short over-all survival (OS). Confluence. The methylation of p16 and / or p15 gen e plays a key role in MM apoptosis path ogenesis.The patients with both p16an d p15gene me thylation had a poor prognosis.