论文部分内容阅读
·AIM:To investigate the effect of hypoxia on the proliferation of mouse corneal epithelial cells in vitro.·METHODS:Mouse corneal epithelial cells(MCEs) were cultured in normoxia (210mL/L O2 and 50mL/L CO2) and hypoxia (20mL/L O2 and 50mL/L CO2),respectively.Colony forming efficiency (CFE) and cell proliferation were determined.The expression of corneal epithelial progenitor cell marker p63 and K19 was investigated by immunostaining.·RESULTS:Normoxic colonies were smaller compared with colonies formed in hypoxia.CFE was (12.50?à1.50)% in hypoxic cultures,which was similar compared with normoxia cultures [(11.13?à1.86)%,P >0.05)].Cell proliferation was enhanced in hypoxia.Progenitor markers p63 and K19 were expressed in most cells under both normoxic and hypoxic conditions.·CONCLUSION:Murine limbal epithelial progenitor cells can be efficiently expanded in hypoxic conditions.·
· METHODS: Mouse corneal epithelial cells (MCEs) were cultured in normoxia (210 mL / L O2 and 50 mL / L CO2) and hypoxia (20 mL / L O2 and 50 mL / L CO2), respectively.Colony forming efficiency (CFE) and cell proliferation were determined. The expression of corneal epithelial progenitor cell marker p63 and K19 was investigated by immunostaining. RESULTS: Normoxic colonies were smaller than colonies formed in hypoxia.CFE was (12.50? a1.50)% in hypoxic cultures, which was similar to normoxia cultures [(11.13? a 1.86)%, P> 0.05)]. Cell proliferation was enhanced in hypoxia. and K19 were expressed in most cells under both normoxic and hypoxic conditions. · CONCLUSION: Murine limbal epithelial progenitor cells can be greatly expanded in hypoxic conditions. ·