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目的分析FLT3基因突变在急性早幼粒细胞白血病(APL)中的临床意义。方法抽取34例APL患者骨髓标本,采用PCR、RT-PCR和DNA酶切技术检测FLT3基因内部串连重复(FLT3-ITD)、FLT3基因D835突变(FLT3-D835),并分析它们与患者临床表现、症状、疗效的关系。结果APL患者外周血高WBC数(WBC≥10×109/L)的阳性率,FLT3-ITD突变型明显高于FLT3-ITD野生型(P<0.05),而FLT3-D835突变与其野生型差异无显著性;骨髓原始细胞+早幼粒细胞之和高含量(≥80%)、B级出血症状、完全缓解(CR)阳性率,FLT3突变型与FLT3野生型患者差异均无统计学意义(P>0.05)。结论大部分FLT3-ITD突变型APL患者常表现高外周血WBC数,骨髓原始细胞与早幼粒细胞之和高含量,B级出血症状及CR率与野生型患者无显著差异。
Objective To analyze the clinical significance of FLT3 gene mutation in acute promyelocytic leukemia (APL). Methods Thirty-four bone marrow samples from patients with APL were collected. FLT3-FLD3-FLT3-FLT3-D835 mutation was detected by PCR, RT-PCR and DNA digestion. FLT3- , Symptoms, the relationship between efficacy. Results The positive rates of WBC (WBC≥10 × 109 / L), FLT3-ITD in APL patients were significantly higher than those in FLT3-ITD wild-type patients (P <0.05), while those in FLT3-D835 patients were not significantly different from those in wild type (P> 0.05) .Conclusion: There is no significant difference between the FLT3 mutant group and FLT3 wild-type patients (P> 0.05) > 0.05). Conclusions Most FLT3-ITD mutant APL patients have high peripheral blood WBC counts, high levels of bone marrow blasts and promyelocytes, grade B hemorrhage and CR rates compared with wild-type patients.